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  • Animal Sterility Laboratory Purification Engineering Design

Animal Sterility Laboratory Purification Engineering Design

According to the different types and different levels of experimental animals, the establishment of the corresponding facilities of the purification of animal housing, activities and the corresponding auxiliary space. The site should be chosen where you can keep quiet, clean, and have no adverse effects. The house must be light enough, well ventilated, clean and tidy, no water; ceiling, walls should be easy to clean, disinfect; external walls, roofs, roofs, doors and windows and outside the pipeline must eliminate external animals, mosquitoes and other pests In addition, the need to establish a different animal laboratory.
  • Case Introduction

Animal Sterility Laboratory Purification Engineering Design

First, according to experimental animal microbiological control standards, the experimental animals can be divided into four levels:
  
Level 1: Common animals (CV), refers to the general control of microorganisms without special control. Requiring the elimination of zoonotic pathogens and the small number of pathogens of experimental animals that are highly infectious. In order to prevent infectious diseases, in the experimental animal breeding and breeding, to take certain measures, should ensure that the results used for testing with the reproducibility of the reaction (that is, regardless of the different operators, at different times, with the same strain Animals can achieve almost the same results by experimenting with prescribed experimental procedures).
  
Level 2: Clean the animal (CL), requiring the elimination of zoonotic and animal pathogens of major infectious diseases.
  
Level 3: No special pathogen (SPF), requiring two to the outside, but also exclude some of the prescribed pathogens. The method of sterilization and sterilization can be carried out using a highly effective air filter sterilization method, an ultraviolet sterilization method, a triethylene glycol vapor spray method and a lithium chloride aqueous solution spray method.
  
Level 4: sterile animal (GF) or schistosomiasis (GN). Sterile animals require no microorganisms that can be detected by existing methods. Somophytes require the implantation of one or several known microorganisms on sterile animals.

动物实验室

 Two, four types of experimental animals also have different pathological examination standards:

   
First appearance of healthy, the main organs should not have lesions.
  
In addition to grade two indicators, microscopic examination of secondary microbial pathogens.
  
Three non-special pathogens. No two, three microbial pathogens.
  
Four does not contain two or three microbial pathogens, spleen, lymph nodes are sterile animal histological structure.
  
In combination with the above, different levels of experimental animals in animal housing design and management have different requirements. Sterile, known bacteria and no special pathogens need to be in a sterile or aseptic environment as possible to keep the environment, the current international common known as the barrier environment, that is, with a barrier to the surrounding animals and the surrounding environment Open, just as fetal rats in the same as the mother's womb. This environment is divided into five categories: isolation system, barrier system, semi-barrier system, open system and laminar flow system from the point of view of controlling microorganism.
  
A isolation system is a system for keeping animals in containers with operating gloves for feeding sterile animals and habitats. Internal maintenance according to microbiological requirements of 100 cleanliness, but its set of rooms and operators do not have to consider the sterile room.
  
B barrier system to 10000 ~ 100000 or so aseptic clean room as a breeding room, mainly for non-special pathogens animal breeding and breeding long。 Into the implementation of strict management, such as shower, for personal clothes and so on.
  
C semi-barrier system to relax the barrier system in the human and objects into the room when the management, the plane composition is roughly the same with the barrier system
  
D laminar flow cage is placed in clean horizontal laminar air. Commonly used in small-scale feeding, but in the general room for breeding, operation and handling of the risk of being contaminated。 Can be used for half-barrier supplement.
  
E open system is on people, objects, air and other rooms are not implemented to eliminate pollution of the system, but usually to a certain degree of clean management.


  Third, the following list of various systems on the experimental animal environmental indicators:

Project / target

Open system(CV)

Simple barrier system(CL)

Barrier system(SPF)

Isolation system(GF,GN)

temperature/℃

18~29

18~29

18~29

18~29

Daily temperature difference/℃

8

3

3

3

Relative humidity/%

40~70

40~70

40~70

40~70

Number of ventilation/(次/h)

10~15

10~15

10~15

 

Airflow speed/(m/s)

0.18

0.18

0.18

 

Pressure difference/Pa

20~50

20~50

20~50

 

Cleanliness / grade

10万

1万

100

 

Number of colonies/[One/(Dish·h)]

12.2

2.45

0.49

 

Ammonia mass concentration/(mg/m3)

14

14

14

14

noise/dB

≤60

≤60

≤60

≤60

Illumination/lx

150~300

150~300

150~300

150~300

Fourth, to achieve the animal room environmental indicators of the purification measures are:
  
A. Air purification system because the animal room is a long time activities of experimental animals, the indoor can not be in the breeding conditions are often fumigation and washing method of sterilization, generally only weak drug disinfectant Sassafras wash, in this case In order to avoid microbial infection, to ensure that air purification is the primary condition;
  
B。 The new wind to prevent cross-contamination and deodorization is two problems, to this end, most of the purification air conditioning system must use a new way of wind, but because the purification system ventilation times, but also a new wind, so energy problems;
  
C. Deodorization is not only necessary for the external environment and the staff, but also the experimental animal itself. Because the indoor microbial contamination in addition to the outside, the animal itself excrement and microbial secondary conversion of ammonia, hydrogen sulfide, alcohol and other gases, is also an important pollutant.
5, animal room general environmental factors Control range:
(1) The temperature target is 21 to 27 ° C。 Pharmacopoeia regulations for the 17 ~ 28 ℃. May vary by animal variety.
(2) Humidity 45% ~ 55%. Ministry of Health provides 40% to 60%.
(3) air flow rate of 10 ~ 25cm / s. Avoid direct hair.
(4) the number of ventilation 6 to 15 times / h. The new wind is 2/3.
(5) pressure clean area positive pressure, negative pressure area.
(6) environmental clean level general animal breeding room is greater than 100000 level.
(7) lighting 150 ~ 300lx (artificial lighting).
(8) noise 40 ~ 50dB (no animal), when animals are 60 dB, the Ministry of Health provides less than 70 dB.
(9) Odor ammonia 20ppm.
Six, the basic requirements of animal room design:
(1) site secluded, health
(2) layout is divided into preparation area, feeding area, experimental area. Requirements flow, logistics, animal flow separately (one-way process); require purification sterilization, pest control.
(3) Building requirements are clean corridors, breeding rooms, pollution corridors and other clean rooms.
(4) air-conditioning system can control the temperature and humidity, air velocity and distribution, a certain amount of ventilation and pressure.
(5) lighting no window animal room using fluorescent lamps, a window animal room can be installed windows to filter ultraviolet light。 Requires 12H bright, 12h dark.
(6) water supply with drinking water and refined water.
Seven, animal room settings:
  
According to the different types and different levels of experimental animals, the establishment of the corresponding facilities of the purification of animal housing, activities and the corresponding auxiliary space。 The site should be chosen where you can keep quiet, clean, and have no adverse effects. The house must be light enough, well ventilated, clean and tidy, no water; ceiling, walls should be easy to clean, disinfect; external walls, roofs, roofs, doors and windows and outside the pipeline must eliminate external animals, mosquitoes and other pests In. Indoor temperature control in the 18 ~ 29 ℃, relative humidity of 40% to 70%, less than 60 dB noise。 Cage animal density can not be too much, to have air, exhaust, cooling and warm equipment. Must have a dedicated sewage, drainage facilities to prevent the spread of pathogens。 All kinds of cages to be regularly cleaned and disinfected, litter to be high temperature and pressure or drug disinfection and timely replacement, so that indoor ammonia concentration of 15mg / m3 below.

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